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MitoSciences (MS1091)

Nitrated Human Liver Homogenate Standard (ab131380)

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Overview

  • Product nameNitrated Human Liver Homogenate Standard
  • Tested applicationsWB, SDS-PAGE more details

    • Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferConstituents: 0.806% Sodium chloride, Proprietary component, 1.5% Dodecyl maltoside
  • Concentration information loading...
  • Reagent notes ab131380 was treated with peroxynitrite which modifies tyrosine by acting as an oxidant to produce 3-nitrotyrosine along proteins in the Human Liver Tissue lysate sample.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab131380 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.

    Nitrated Human Liver Homogenate Standard images

    • Western blot - Human Liver Tissue Lysate (ab131380)
      Western blot - Human Liver Tissue Lysate (ab131380)
      All lanes : Anti-Nitrotyrosine antibody ab110282 at 0.4 µg/ml

      Lane 1 : Molecular weight marker
      Lane 2 : Human Liver Homogenate at 10 µg
      Lane 3 : Human Liver Homogenate at 2.5 µg
      Lane 4 : Nitrated Human Liver Homogenate at 10 µg
      Lane 5 : Nitrated Human Liver Homogenate at 5 µg
      Lane 6 : Nitrated Human Liver Homogenate at 2.5 µg
      Lane 7 : Nitrated Human Liver Homogenate at 1.25 µg
      Lane 8 : Nitrated Human Liver Homogenate at 0.625 µg

      Secondary
      Goat polyclonal to Mouse IgG, Pre-Adsorbed (HRP) at 1/6000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes
    • SDS-PAGE - Human Liver Tissue Lysate (ab131380)
      SDS-PAGE - Human Liver Tissue Lysate (ab131380)

      Lane 1: Molecular Weight Marker
      Lane 2: Nitrated Human Liver Homogenate (10 µg)
      Lane 3: Human Liver Homogenate (10 µg)

      Gel: 10-20% acrylamide separation gel

    References for Nitrated Human Liver Homogenate Standard (ab131380)

    ab131380 has not yet been referenced specifically in any publications.

    Publishing research using ab131380 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab131380 Submit a Question for ab131380

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    In need of 5 human normal liver lysates taken from different donors.

    Thank you very much for your call yesterday and for your patience while I've looked into your enquiry.

    We have 5 liver lysates that are from different donors:

    Ab44563-

    http://www.abcam.com/Liver-Human-Tissue-Lysate-adult-norm...

    Read More

    Not seeing any bands at 100kDa. Non specific bands at 50/75kDa.

    Thank you for calling Abcam today.

    I am sorry about the issues that you have been having with ab13868 in western blot. AS we discussed today, I am sending you a vial of human liver lysate to use as a positive control (although I am sending ab2988...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"