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ab197453 can be used for quantitative measurement of 10 Human MMPs. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target names: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4.
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
|Components||8 tests||22 tests||50 tests|
|20X Wash Buffer I||2 x 30ml||3 x 30ml||6 x 30ml|
|20X Wash Buffer II||1 x 30ml||1 x 30ml||2 x 30ml|
|Cy3 equivalent dye-conjugated Streptavidin||1 x 5µl||2 x 5µl||4 x 5µl|
|Human MMP Array (10 T) Biotinylated Antibody Cocktail||1 unit||2 units||4 units|
|Human MMP Array (10 T) Glass Slide||1 slide||2 slides||4 slides|
|Human MMP Array (10 T) Lyophilized Cytokine Standard Mix||1 vial||1 vial||2 vials|
|Sample Diluent||1 x 15ml||1 x 15ml||2 x 15ml|
|Slide Washer/Dryer (30 mL Centrifuge Tube)||1 unit||1 unit||2 units|
ab197453 has not yet been referenced specifically in any publications.