Human MMP10 ELISA Kit (ab100602)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.06 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MMP10 ELISA Kit
See all MMP10 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 3 pg/ml -
Range
2.06 pg/ml - 1500 pg/ml -
Recovery
96 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.31 87% - 109% Serum 96.54 85% - 108% Plasma 94.57 84% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s MMP10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernates.
This assay employs an antibody specific for Human MMP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human MMP10 2 vials MMP10 Microplate (12 x 8 wells) 1 unit Recombinant Human MMP10 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Matrix metallopeptidase 10 (stromelysin 2)
- Matrix metalloprotease 10
- Matrix metalloproteinase 10
see all -
Database links
- Entrez Gene: 4319 Human
- Omim: 185260 Human
- SwissProt: P09238 Human
- Unigene: 2258 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100602 has not yet been referenced specifically in any publications.