Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

Overview

  • Product nameHuman Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105)
  • Product overviewab93758 is a Human Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 mouse monoclonal, 50µg of CD45 rabbit polyclonal, 50µg of CD90 mouse monoclonal, 50µl of CD29 rabbit monoclonal and 50µl of CD105 mouse monoclonal antibodies.
    The Human Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated human multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD105, CD29, CD44, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.
  • Components

      Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) images

      • ICC/IF image of ab52971 staining CD29 in Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
      • Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10559 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive. Based on the accompanying image, approximately 9.62% of cells exhibited positive staining for anti-CD45. Since KM-H2 have low levels of CD45 transcripts, it is expected that they have low levels of CD45 on their surface which is reflected in the ~9% positive. This image is taken from an Abreview.
      • Ab6124 staining CD44 on Human Tissue sections (Skin tumor) by IHC-P. Sections were Formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid prior to blocking with 10% serum for 30 mins at 25°C. The primary antibody was diluted 1/100 in PBS and incubated with the sample overnight at 4°C. A TRITC non-Abcam secondary antibody was used.

      References for Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)

      This product has been referenced in:
      • Chen TT  et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). Read more (PubMed: 20176926) »
      • Chang HL  et al. Uterine leiomyomas exhibit fewer stem/progenitor cell characteristics when compared with corresponding normal myometrium. Reprod Sci 17:158-67 (2010). Read more (PubMed: 19805552) »

      See all 3 Publications for this product

      Product Wall


      ab93758 consists fo 5 vials of different antibodies.
      Anti-CD44 antibody [F10-44-2](ab 6124 ) Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP Mouse monoclonal (50µg ) 1mg/ml Reacts with: Hu
      Anti-CD45 antibody(ab 10559 ) Flow Cyt, ICC, IHC-Fr, IHC-P, ...

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      Thank you for contacting Abcam.
      Earlier today we discussed the Human Mesenchymal Stromal Cell Marker panel ab93758. Based on the information on the datasheet, I compiled the following information for you:
      1. Positive selection (CD105, CD29, CD4...

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      Thank you for your enquiry and your interest in our products.

      ab93758 is a Human Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 mouse monoclonal, 50µg of CD45 rabbit polyclonal, 50µg of CD90 mouse monoclonal, ...

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      Vielen Dank für Ihre Antwort.
      Ich entschuldige mich für die Verwirrung. Ja, die Antikörper werden die Oberflächenproteine markieren. Ich habe nur den Fakt angesprochen, dass, wenn Sie Schnitte machen, die Zellen ja aufgeschnitten sind. Aber in bei...

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      Vielen Dank für Ihre Email.
      Ja, viele Forscher machen semi-quantitive Analysen basieren auf Immunhistofluorsezenz. Hier sind Links zu Artikel, die dies diskutieren:
      http://www.nature.com/protocolexchange/protocols/2349
      http://onlinelibra...

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      Thank you for your enquiry and interest in our products.

      I can confirm that this panel contains 5 primary antibodies which recognize human mesenchymal stromal cells: ab6124, ab10559, ab23894, ab44967, ab52971. The datasheets can be found at t...

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      Thank you for contacting Abcam earlier today.

      I am sorry that the vials of CD44 and CD45 were shot when they arrived. As we discussed we will send out free of charge replacements for these two antibodies to make up for this shortage. Your new...

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      Thank you for your inquiry.
      The stem cell panel ab93758 consists of 5 unconjugated primary antibodies. These antibodies can be used with the secondary antibodies against mouse or rabbit of your choice. You can therefore adapt the fluorochrome choi...

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      Thank you for your inquiry.

      Our Mesenchymal Stromal Cell Marker Panels (human: ab93758, mouse: ab93759) consist of five un-conjugated primary antibodies that can be used to characterise mesenchymal stromal cells.

      The antibodies are ...

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      Vielen Dank für Ihren Anruf vorhin. Ich habe mich nun erkundigt und kann Ihnen nochmals bestätigen, dass diese Panel nicht getestet ist um alle Färbungen auf einmal zu machen. Dieses Panel wurde zusammengestellt um unseren Kunden kleinere Größen von An...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"