Product overviewab93758 is a Human Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 mouse monoclonal, 50µg of CD45 rabbit polyclonal, 50µg of CD90 mouse monoclonal, 50µl of CD29 rabbit monoclonal and 50µl of CD105 mouse monoclonal antibodies.
The Human Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated human multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD105, CD29, CD44, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.
Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) images
Immunocytochemistry/ Immunofluorescence - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
ICC/IF image of ab52971 staining CD29 in Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52971, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
Asynchronous KM-H2 cells were pelleted and labeled by indirect immunofluorescence. Cells were stained with ab10559 (1/200) for 30min at 4'C, washed and then stained with goat anti-rabbit alexafluor 488 (1/200). Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive. Based on the accompanying image, approximately 9.62% of cells exhibited positive staining for anti-CD45. Since KM-H2 have low levels of CD45 transcripts, it is expected that they have low levels of CD45 on their surface which is reflected in the ~9% positive. This image is taken from an Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
Ab6124 staining CD44 on Human Tissue sections (Skin tumor) by IHC-P. Sections were Formaldehyde fixed and subjected to heat mediated antigen retrieval in citric acid prior to blocking with 10% serum for 30 mins at 25°C. The primary antibody was diluted 1/100 in PBS and incubated with the sample overnight at 4°C. A TRITC non-Abcam secondary antibody was used.
References for Human Mesenchymal Stromal Cell Marker Panel (CD44, CD45, CD90 CD29 and CD105) (ab93758)
This product has been referenced in:
Chang HL et al. Uterine leiomyomas exhibit fewer stem/progenitor cell characteristics when compared with corresponding normal myometrium. Reprod Sci17:158-67 (2010).
Read more (PubMed: 19805552) »
Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol188:595-609 (2010).
Read more (PubMed: 20176926) »