![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free (ab80650) image](#Human-Milk-Fat-Globule-1-Primary-antibodies-ab80650-1.jpg)
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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free
See all Human Milk Fat Globule 1 products (6) ...
Description
Mouse monoclonal [EDM45] to Human Milk Fat Globule 1 - BSA and Azide free
Specificity
We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IP, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Delipidated human milk fat globule membrane preparation.
Positive control
SKBR-3 cells. Breast, colon, or lung tissues.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab80650 is purified from ascites fluid by Protein G chromatography.
Clonality
Monoclonal
Clone number
EDM45
Isotype
IgG1
Light chain type
kappa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free (ab80650)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free (ab80650) image](/ps/datasheet/images/80/ab80650/Human-Milk-Fat-Globule-1-Primary-antibodies-ab80650-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab80650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 43 kDa.
IP: Use at 2 µg/mg of lysate.
IHC-P: Use a concentration of 1 - 2 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. MFG1 Binds specifically to rotavirus and inhibits its replication.
Cellular localization
Cell Membrane and Secreted
Alternative names
- BA 46 antibody
- BA46 antibody
- Breast epithelial antigen BA46 antibody
see all
Database links
- Entrez Gene: 4240 Human
- Omim: 602281 Human
- SwissProt: Q08431 Human
- Unigene: 3745 Human
Anti-Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free (ab80650)
ab80650 at 1µg/ml staining Human Milk Fat Globule 1 in human colon by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epithelial cells. Staining was performed using antibody containing BSA and azide.
References for Anti-Human Milk Fat Globule 1 antibody [EDM45] - BSA and Azide free (ab80650)
ab80650 has not yet been referenced specifically in any publications.
Publishing research using ab80650? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"