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IHC-P - Human Milk Fat Globule 1 antibody [SPM291]
![IHC-P - Human Milk Fat Globule 1 antibody [SPM291]](/ps/datasheet/images/ab17787_1.jpg)
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Product Name
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Human Milk Fat Globule 1 antibody [SPM291]
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See all Human Milk Fat Globule 1 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [SPM291] to Human Milk Fat Globule 1
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Immunogen
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Delipidated human milk fat globule membrane preparation.
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Reacts with
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Hu
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Does not react with
(species key)
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Rat
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at an assay dependent dilution.
IHC-P: 1/50 (30 min at RT). Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min..
IP: Use 2µg/mg of lysate.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 40-45 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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SKBR-3 cells. Breast, colon, or lung tissues.
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Cellular localization
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Cell Membrane and Secreted
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Research areas
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Developmental Biology >> Reproduction >> Fertilisation
Cardiovascular >> Lipids / Lipoproteins >> Adipose Related >> Lipid Droplet Protein
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Relevance
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Human Milk Fat Globule 1 (MFG1) is a membrane protein present on normal human breast epithelial cells and cell lines derived from breast carcinomas. Human MFG1 is also localised on the surface of the human milk fat globule. HMFG1 is a differentiation marker and specific breast epithelial marker in normal and neoplastic mammary development. MFG1 Binds specifically to rotavirus and inhibits its replication.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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SPM291
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Human Milk Fat Globule 1 antibody [SPM291] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Human Milk Fat Globule 1 antibody [SPM291] images, references, products related to ab17787 and other tools.
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Human Milk Fat Globule 1 antibody [SPM291] images:
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 IHC-P - Human Milk Fat Globule 1 antibody [SPM291]
ab17787 Milk Fat Globule Membrane Protein staining in human (1/50, following 30min incubation at RT).
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Search PubMed (MEDLINE) for references to Human Milk Fat Globule 1
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Human Milk Fat Globule 1 antibody [SPM291] - more information
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Customer reviews (feedback) regarding Human Milk Fat Globule 1 antibody [SPM291] |
Customer FAQs regarding Human Milk Fat Globule 1 antibody [SPM291] |
Protocols for Human Milk Fat Globule 1 antibody [SPM291] |
Price and availability of products related to Human Milk Fat Globule 1 antibody [SPM291] |
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Human Milk Fat Globule 1 antibody [SPM291] - related products:
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Human Milk Fat Globule 1 antibody [SPM291] - other tools:
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Contact Abcam with a Technical Enquiry about Human Milk Fat Globule 1 antibody [SPM291] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![IHC-P - Human Milk Fat Globule 1 antibody [SPM291]](/index.html?datasheet=17787#ab17787_1.jpg)
