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Abcam’s anti-Mumps virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Mumps virus in Human serum and plasma.
A 96-well plate has been precoated with Mumps virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Mumps virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mumps virus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Mumps virus (IgG) Coated Microplate (12 x 8 wells)||12 x 8 well Strips||1 unit|
|Mumps virus anti-IgG HRP Conjugate||Blue, Black cap||1 x 20ml|
|Mumps virus IgG Cut-off Control||Yellow, Green cap||1 x 3ml|
|Mumps virus IgG Negative Control||Yellow, Blue cap||1 x 2ml|
|Mumps virus IgG Positive Control||Yellow, Red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108752 in the following tested applications.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108752 has not yet been referenced specifically in any publications.