Overview

  • Product name
    Human Myeloperoxidase ELISA Kit
    See all Myeloperoxidase kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 2.1%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.5 pg/ml
  • Range
    15.6 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108 105% - 112%
    Saliva 103 96% - 108%
    Milk 114 107% - 121%
    Urine 104 101% - 105%
    Serum 104 102% - 106%
    Heparin Plasma 98 92% - 106%
    EDTA Plasma 70 64% - 76%
    Citrate Plasma 108 103% - 117%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Myeloperoxidase (MPO) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Myeloperoxidase protein in human serum, plasma, cell culture supernatant, urine, milk, saliva, cell and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
    Sensitivity:


    Samples diluted in Sample Diluent NS – 2.5 pg/mL
    Samples diluted in 1X Cell Extraction Buffer – 5.9 pg/mL

  • Notes

    Human Myeloperoxidase (MPO) is a hemoprotein expressed in the neutrophils (polymorphonuclear leukocytes) and is secreted during activation. Myeloperoxidase catalyzes the oxidation of chloride ions to hypochlorus acid, which is a potent antimicrobial agent. Myeloperoxidase pro-oxidative and pro-inflammatory properties cause an increase in MPO in multiple diseases including heart disease, myocardial infraction, and multiple sclerosis. Myeloperoxidase is a clinical marker of myocardial infraction and cardiovascular disease.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab195212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Myeloperoxidase is released from neutrophils upon exposure to activated platelets. Use platelet poor plasma’s for measuring circulating levels of Myeloperoxidase. The concentrations of Myeloperoxidase were measured in duplicate and interpolated from the Myeloperoxidase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of Myeloperoxidase were measured in duplicate and interpolated from the Myeloperoxidase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myeloperoxidase concentration was determined to be 1042 ng/mL in Human saliva.

  • PBMC were grown in the absence (unstimulated) or presence of phytohemagglutinin (PHA) (stimulated) for 2 days. Myeloperoxidase was measured in 2-fold diluted cell culture supernatants of unstimulated and PHA stimulated PBMC and media control. Measured values were interpolated from the Myeloperoxidase Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 162 ng/mL unstimulated, 183 ng/mL stimulated, and undetectable in media.

Protocols

References

ab195212 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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