Human NGF R ELISA Kit (CD271) (ab155436)
Key features and details
- Sensitivity: 80 pg/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NGF R ELISA Kit (CD271) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Intra-assay < 10% Inter-assay Sample n Mean SD CV% Inter-assay < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 80 pg/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 86.88 79% - 95% Serum 82.75 75% - 93% Plasma 84.17 76% - 93% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s NGF R (CD271) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human NGF R (CD271) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human NGF R coated on a 96-well plate. Standards and samples are pipetted into the wells and NGF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NGF R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NGF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
The standard is affinity purified.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human NGF R 2 vials NGF R Microplate (12 x 8 wells) 1 unit Recombinant Human NGF R Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Alternative names
- CD271
- Gp80-LNGFR
- Low affinity neurotrophin receptor p75NTR
see all -
Database links
- Entrez Gene: 4804 Human
- Omim: 162010 Human
- SwissProt: P08138 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155436 has been referenced in 1 publication.
- Jiang H et al. The serum protein levels of the tPA-BDNF pathway are implicated in depression and antidepressant treatment. Transl Psychiatry 7:e1079 (2017). ELISA . PubMed: 28375203