Overview

  • Product name
    Human PD-L1 ELISA Kit [28-8]
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.1%
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    2.91 pg/ml
  • Range
    21.87 pg/ml - 1400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 102% - 105%
    Urine 91 86% - 96%
    Serum 86 82% - 91%
    Cell culture extracts 102 100% - 103%
    Tissue Extracts 105 104% - 107%
    Heparin Plasma 92 89% - 93%
    EDTA Plasma 85 81% - 88%
    Citrate Plasma 84 79% - 88%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
  • Product overview

    PD-L1 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PD-L1 protein in serum, plasma, and cell culture supernatant, cell and tissue extracts.


    We combined our highly specific RabMAb PD-L1 antibody [clone 28-8] (ab205921) with our 90-minute SimpleStep ELISA protocol to create a kit with nearly 2-fold higher sensitivity than our main competitor’s published results.  


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples in Sample Diluent NS: 2.16 pg/mL


    Samples in 1X Cell Extraction Buffer PTR: 8.97 pg/mL

  • Notes

    PD-L1 (also known as CD274 or B7-H1) is a membrane bound glycoprotein involved in regulation of the immune system. PD-L1 is expressed on a variety of inflammatory-activated cells as well as some carcinomas and in melanoma. PD-L1 binds to PD-1 and CD80, where it can suppress T cell activation and proliferation as well as induce apoptosis. Levels of PD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. PD-L1 can bind PD-1 in order to regulate T cell apoptosis.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Function
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity
    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane and Endomembrane system.
  • Information by UniProt
  • Alternative names
    • B7 H
    • B7 H1
    • B7 homolog 1
    • B7-H1
    • B7H
    • B7H1
    • CD 274
    • CD274
    • CD274 antigen
    • CD274 molecule
    • MGC142294
    • MGC142296
    • OTTHUMP00000021029
    • PD L1
    • PD-L1
    • PD1L1_HUMAN
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL 1
    • PDL1
    • Programmed cell death 1 ligand 1
    • Programmed death ligand 1
    • RGD1566211
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab214565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human PD-L1 ELISA Kit [28-8] images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 404.3 pg/mL in Jurkat stimulated with LPS and IFN-gamma, 628.4 pg/mL in placenta and 207.5 pg/mL in thyroid extracts.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  PD-L1 was measured in 6 donor serum samples and the remaining 4 samples measured less than the lowest point of the PD-L1 standard curve. Of those measured, the mean PD-L1 concentration was determined to be 44.5 pg/mL with a range of 34.4 – 75.3 pg/mL.

  • Conditioned media was harvested after 48 hours. PD-L1 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 59.7 pg/mL in PHA stimulated PBMC supernatant. There was no detectable signal in unstimulated supernatant.

Protocols

References for Human PD-L1 ELISA Kit [28-8] (ab214565)

ab214565 has not yet been referenced specifically in any publications.

Product Wall

We refer to sensitivity as the "Minimal Detectable Dose" (MDD) which is defined as follows on page 19 of the protocol booklet linked to the http://www.abcam.com/human-pd-l1-elisa-kit-28-8-ab214565.html near the upper right.


From page 19 o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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