Recombinant Human PLA2G1B protein (ab63842)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB
Description
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Product name
Recombinant Human PLA2G1B protein
See all PLA2G1B proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHH GMASHMAVWQ FRKMIKCVIP GSDPFLEYNN YGCYCGLGGS GTPVDELDKC CQTHDNCYDQ AKKLDSCKFL LDNPYTHTYS YSCSGSAITC SSKNKECEAF ICNCDRNAAI CFSKAPYNKA HKNLDTKKYC QS -
Amino acids
23 to 148 -
Tags
His tag N-Terminus -
Additional sequence information
Total 142 AA. MW: 16 kDa (calculated). N-Terminal His-tag, 16 extra AA
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab63842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Lyophilized -
Additional notes
Product is NOT sterile! Please filter the product by an appropriate sterile filter before using it in cell culture. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.41% Sodium acetate
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ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited.
General Info
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Alternative names
- Group IB phospholipase A2
- Group IB secretory phospholipase A2
- MGC119834
see all -
Function
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. -
Sequence similarities
Belongs to the phospholipase A2 family. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab63842 has not yet been referenced specifically in any publications.