Human SDF1 alpha + SDF1 beta ELISA Kit (ab100637)
Key features and details
- Sensitivity: 42 pg/ml
- Range: 42 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SDF1 alpha + SDF1 beta ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 42 pg/ml -
Range
42 pg/ml - 6000 pg/ml -
Recovery
> 80 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.63 86% - 104% Plasma 89.34 85% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human SDF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100637) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human SDF-1 alpha in normal serum with ab100637, only in serum spiked with Human SDF-1 alpha.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human SDF1 alpha 2 vials Recombinant Human SDF1 alpha Standard (lyophilized) 2 vials SDF1 alpha Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Cellular localization
SDF1 beta: Secreted. -
Database links
- Entrez Gene: 6387 Human
- Omim: 600835 Human
- SwissProt: P48061 Human
- SwissProt: P48061 Human
- Unigene: 522891 Human
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab100637 has been referenced in 14 publications.
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e54271 (2022). PubMed: 35403791
- Lu X et al. CXCL12 secreted by pancreatic stellate cells accelerates gemcitabine resistance of pancreatic cancer by enhancing glycolytic reprogramming. Anim Cells Syst (Seoul) 26:148-157 (2022). PubMed: 36046033
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e55871 (2022). PubMed: 36200589
- Liu L et al. Enhanced Effect of IL-1ß-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling. Stem Cells Int 2020:2803747 (2020). PubMed: 32377202
- Yang X et al. Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell. Medicine (Baltimore) 99:e20944 (2020). PubMed: 32629699