Overview

  • Product name
    Human TGF beta 1 ELISA Kit
    See all TGF beta 1 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 80 pg/ml
  • Range
    0.082 ng/ml - 60 ng/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.87 85% - 104%
    Serum 94.46 82% - 102%
    Plasma 95.78 93% - 103%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TGF beta 1 (TGFB1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human TGF beta 1 in serum, plasma, cell culture supernatants.

    Many cells synthesize TGFβ1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

    This assay employs an antibody specific for human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

     

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TGF beta 1 (lyophilized) 2 vials
    Recombinant Human TGF beta 1 Standard 2 vials
    Stop Solution 1 x 8ml
    TGF beta 1 Microplate (12 x 8 wells) 1 unit
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • Tissue specificity
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • Involvement in disease
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • Sequence similarities
    Belongs to the TGF-beta family.
  • Post-translational
    modifications
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • Camurati-Engelmann disease
    • CED
    • DPD1
    • LAP
    • Latency-associated peptide
    • TGF beta 1 protein
    • TGF-beta-1
    • TGFB
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • Transforming growth factor beta 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve in Assay Diluent A : mean of duplicates (+/-SD)

  • Standard curve in Assay Diluent B : mean of duplicates (+/-SD)

Protocols

References

This product has been referenced in:
  • Recha-Sancho L & Semino CE Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering. PLoS One 11:e0157603 (2016). Read more (PubMed: 27315119) »
  • Tian Y  et al. Serum deprivation response inhibits breast cancer progression by blocking transforming growth factor-ß signaling. Cancer Sci 107:274-80 (2016). Read more (PubMed: 26749136) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. 1. Here is a link to a kit that will detect human IL17F: http://www.abcam.com/Human-IL17F-ELISA-Kit-1-x-96-Well-Plate-ab100557.html Tested samples: Cell culture supernatant, Urine, Serum, Plasma Cost: £335.00 2. Here is a li...

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Thank you for your enquiry. i) Is this kit can measure the cytokines in bronchoalveolar lavage (BAL) samples? Or do you have any other recommendation for measurement of TGF beta 1 in BAL samples.  Thusfar this kit has been used to detect TGF beta in ce...

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Thank you for your email. We have ab100647 in stock. I have also requested our datasheet department for addition of a ab65355 standard as separate product in catalogue. I am now waiting a reply from them. I will be updating you further once this...

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Thank you for answeirng my questions. Ab10647 have HRP standard 8ul vial and ab46087 have 2 vial of 4ul each. The amount of HRP-Streptavidin in these vials is very-very small; might not be visible. We would recommend following the datasheets rec...

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Thank you for contacting us. I apologize for the delay in response. I will try to solve this case asap. I have read your email which is slightly confusing so I would appreciate if you could answer my following questions. - Could you plea...

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11-15 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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