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Question (44623) | Human TGF beta 1 ELISA Kit (ab100647)

The Assay Diluent B 5x was cloudy upon arrival. Is this normal or is the sample contaminated?

Thank you for contacting Abcam regarding ab100647.

I have received some further information from the lab regarding this issue and I wanted to share this with you.

The appearance of Assay Diluent B can vary by batch but a little cloudiness or precipitate is not something to be concerned with as it does contain protein and other solutes.While the solution appearance may look visibly unappealing, there is nothing based on our testing and customer feedback that says the assay or more importantly the data would be negatively affected. I would encourage the customer to bring the solution up to room temperature, mix thoroughly and dilute to the 1X working solution to help clarify the solution and incorporate any protein aggregates.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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