Overview

  • Product name
    Human TNFRSF14 ELISA Kit
    See all TNFRSF14 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 4.5%
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    9.2 pg/ml
  • Range
    31.3 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 107 103% - 113%
    Cell culture extracts 105 100% - 109%
    Tissue Extracts 105 103% - 106%
    Cell culture media 116 114% - 119%
    Heparin Plasma 102 98% - 105%
    EDTA Plasma 102 91% - 110%
    Citrate Plasma 107 100% - 115%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    TNFRSF14 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human TNFRSF14 protein in serum, plasma, cell culture supernatant, cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 13.5 pg/mL.
    Samples in 1X Cell Extraction Buffer PTR: 9.2 pg/mL.

  • Notes

    TNFRSF14 (also known as tumor necrosis factor receptor superfamily member 14 or Herpesvirus entry mediator or HVEM) is a cell surface receptor of the TNF-receptor superfamily.  TNFRSF14 is a receptor for BTLA, LIGHT, lymphotaxin alpha. Herpesvirus glycoprotein D binds to TNFRSF14.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human TNFRSF14 Capture Antibody 1 x 600µl
    10X Human TNFRSF14 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    ab221825 - Antibody Diluent 5BI 1 x 6ml
    Human TNFRSF14 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas
  • Function
    Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
  • Tissue specificity
    Widely expressed, with the highest expression in lung, spleen and thymus.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • HVEML
    • ATAR
    • CD270
    • CD40 like protein precursor
    • Herpes virus entry mediator A
    • Herpesvirus entry mediator
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HveA
    • HVEM
    • HVEM L
    • LIGHT
    • LIGHTR
    • TNFRSF14
    • TNFSF 14
    • TNR14_HUMAN
    • TR2
    • Tumor necrosis factor receptor like gene2
    • Tumor necrosis factor receptor superfamily member 14
    • Tumor necrosis factor receptor superfamily member 14 precursor
    • Tumor necrosis factor receptor-like 2
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab216950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of TNFRSF14 were measured in duplicates, interpolated from the TNFRSF14 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2,507 pg/mL in serum, 2,050 pg/mL in plasma (citrate) and 1,696 pg/mL in plasma (EDTA) and 2,044 pg/mL in plasma (heparin).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 2413 pg/mL with a range of 1201 – 4953 pg/mL.

  • PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TNFRSF14 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated PBMC and cell culture media.  Measured values were interpolated from the TNFRSF14 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor.  Mean of duplicate values +/-SD are graphed: 211 pg/mL  in unstimulated, 744 pg/mL in stimulated, and undetectable in media.

  • Samples based on a 400 μg/mL extract load. The concentrations of TNFRSF14 were measured in duplicate and interpolated from the TNFRSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFRSF14 concentration was determined to be 306 pg/mL in Raji Extract, 345 pg/mL in HL-60 extract, and 809 pg/mL in Human Liver Homogenate Extract.

Protocols

References

ab216950 has not yet been referenced specifically in any publications.

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