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Human TRAIL (CD253) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab46074) is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.
A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.
This kit will recognize both endogenous and recombinant Human TRAIL.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|10X Standard Diluent Buffer||Black||1 x 25ml||1 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated Antibody Diluent||Red||1 x 7ml||1 x 13ml|
|Biotinylated anti-TRAIL||Red||1 x 400µl||2 x 400µl|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|Control||Silver||2 vials||4 vials|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|Plastic Plate Covers||2 units||4 units|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin-HRP||2 x 5µl||4 x 5µl|
|TRAIL Microplate (12 x 8 well strips)||1 unit||2 units|
|TRAIL Standard (Lyophilized)||Yellow||2 vials||4 vials|
Our Abpromise guarantee covers the use of ab46074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
TRAIL measured in undiluted biological fluids (duplicates +/- SD).
Representative Standard Curve using ab46074
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"