Human VCAM-1 ELISA Set (without plates) (ab47355)
Overview
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Product name
Human VCAM-1 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.6 ng/ml -
Range
1.56 ng/ml - 50 ng/ml -
Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human VCAM1
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 10 x 96 tests 15 x 96 tests 5 x 96 tests Chromogen TMB Substrate Solution 4 vials 6 vials 2 vials Detection biotinylated anti-VCAM-1 antibody 2 x 0.55ml 3 x 0.55ml 1 x 0.55ml Streptavidin-HRP 2 vials 3 vials 1 vial VCAM-1 Capture Antibody 2 x 0.5ml 3 x 0.5ml 1 x 0.5ml VCAM1 standard 10 vials 15 vials 5 vials -
Research areas
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Relevance
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- CD106
- CD106 antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 7412 Human
- SwissProt: P19320 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab47355 has been referenced in 3 publications.
- Meneses GC et al. Endothelial biomarkers as predictors for haemodialysis need in severe leptospirosis patients (Weil's disease). Trop Med Int Health 27:727-734 (2022). PubMed: 35761507
- Tessarolo LD et al. Endothelial activation is associated with albuminuria in multibacillary leprosy. Rev Inst Med Trop Sao Paulo 63:e44 (2021). PubMed: 34161550
- Lino DOC et al. Interleukin-6 and adhesion molecules VCAM-1 and ICAM-1 as biomarkers of post-acute myocardial infarction heart failure. Braz J Med Biol Res 52:e8658 (2019). PubMed: 31778438