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GOod morning, I am interested in buying in the future a VEGF elisa kit. I received an email from your company, showing such a product (catalog # ab 100663) and I noticed it measures VEGF in cell/tissue extracts. as other kits use instead cell supernatants, I was wondering if you could comment on this, i.e. advantages, if the internal level reflects the secreted levels (as there are also membrane retained forms) and so on... I am working on a protein that may change VEGF levels, acting through modulation of HIF1alpha stability. Looking forward to your answerr Sincerely
Asked on Nov 16 2011
Thank you for your inquiry and your interest in our products. ab100663 has been specially designed and optimized for cell and tissue lysate samples. If you would like to use different type of sample, I would advise consideraing the serum, plasma, and cell culture supernatant Human VEGF ELISA Kit (ab100662) instead. If you need any further assistance in the future, please do not hesitate to contact me.
Abcam Scientific Support
Answered on Nov 16 2011