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I would like to order the VEGF Human Elisa kit ab100663 and to use it on protein extracts in lysis buffer countaining 8M urea . Do you know if the elisa should work with Urea 1M after dillution in the sample Diluent Buffer.
Thanks a lot
Asked on Sep 25 2012
Thank you for your enquiry.
I am sorry to confirm we have not tested the effects of Urea containing lysis buffer on samples tested with ab100663 VEGF Human ELISA Kit. We would therefore not be able to confirm how well this would work. However, it is important to note that Urea is a Chaotropic agent at 6 - 8 M, meaning that it can denature proteins. Therefore this buffer formula may adversely affect the VEGF protein, causing it not to be recognized by the ELISA.
I am sorry we have no data to help answer your query on this occasion, however I hope the information is helpful. If you have any further questions, please do not hesitate to contact us.
Abcam Scientific Support
Answered on Sep 25 2012