Overview

  • Product name
    Human beta IG H3 ELISA Kit (TGFBI)
    See all IGH3 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type
    Serum, Plasma, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    0.025 ng/ml - 6 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 92.44 76% - 113%
    Plasma = 86.91 69% - 104%
    Cell culture media = 104.8 96% - 114%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s beta IG H3 (TGFBI) Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human beta IG H3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human beta IG H3 (TGFBI) coated on a 96-well plate. Standards and samples are pipetted into the wells and beta IG H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta IG H3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta IG H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization maybe required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Alternative names
    • Beta ig-h3
    • beta-IG-H3
    • BIGH3
    • CDB1
    • CDG2
    • CDGG1
    • CSD
    • CSD1
    • CSD2
    • CSD3
    • EBMD
    • Kerato-epithelin
    • LCD1
    • RGD-CAP
    • RGD-containing collagen-associated protein
    • TGFBI
    • Transforming growth factor-beta-induced protein ig-h3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab155426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human beta IG H3 ELISA Kit (TGFBI) images

  • Representative standard curve using ab155426 -  Assay Diluent B

Protocols

References for Human beta IG H3 ELISA Kit (TGFBI) (ab155426)

ab155426 has not yet been referenced specifically in any publications.

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