|
|
|
|
Immunocytochemistry/ Immunofluorescence - Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)
![Immunocytochemistry/ Immunofluorescence - Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)](/ps/datasheet/images/ab11396_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Human placental lactogen (epitope 2) antibody [INN-hPL-37]
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [INN-hPL-37] to Human placental lactogen (epitope 2)
|
|
Immunogen
|
Human placental lactogen.
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
Radioimmunological direct binding studies were performed with 125I-labelled. hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1%. Direct binding demonstrated 39% reactivity with hGH but no cross reactivity with hPRL.
|
|
Tested applications
(see key)
|
RIA
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
RIA: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Signal Transduction >> Growth Factors/Hormones >> Hormones
|
|
Relevance
|
Placental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
|
|
|
Alternative names Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396) |
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
INN-hPL-37
|
|
Myeloma
|
x63-Ag8.653
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide.
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purification by affinity chromatography.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Human placental lactogen (epitope 2) antibody [INN-hPL-37] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Human placental lactogen (epitope 2) antibody [INN-hPL-37] images, references, products related to ab11396 and other tools.
|
| |
Human placental lactogen (epitope 2) antibody [INN-hPL-37] images:
|
 Immunocytochemistry/ Immunofluorescence - Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)
ab11396 at 1/100 staining human BeWo cells (placenta, choriocarcinoma) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary antibody. The image is shown in the upper right hand panel. In this experiment ab15554 was used as a positive control (bottom left hand panel). Cells were counter stained with DAPI (upper left hand panel). This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov
See Abreview
|
| |
| |
|
Search PubMed (MEDLINE) for references to Human placental lactogen (epitope 2)
|
| |
Human placental lactogen (epitope 2) antibody [INN-hPL-37] - more information
|
Customer reviews (feedback) regarding Human placental lactogen (epitope 2) antibody [INN-hPL-37] |
Customer FAQs regarding Human placental lactogen (epitope 2) antibody [INN-hPL-37] |
Protocols for Human placental lactogen (epitope 2) antibody [INN-hPL-37] |
Price and availability of products related to Human placental lactogen (epitope 2) antibody [INN-hPL-37] |
| |
Human placental lactogen (epitope 2) antibody [INN-hPL-37] - related products:
|
|
|
| |
Human placental lactogen (epitope 2) antibody [INN-hPL-37] - other tools:
|
Contact Abcam with a Technical Enquiry about Human placental lactogen (epitope 2) antibody [INN-hPL-37] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)](/index.html?datasheet=11396#ab11396_1.jpg)
