You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Huntingtin antibody [1A771] (ab13583)

CodeSizePriceAbpointsAvailability
     
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Huntingtin antibody [1A771]
See all Huntingtin products (13) ...

Description

Mouse monoclonal [1A771] to Huntingtin

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

The antibody was raised against a fusion protein containing 62 glutamine amino acid repeats.

Positive control

Lymphoblast cell line extracts.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

1A771

Isotype

IgG1

  • Western blot - Huntingtin antibody [1A771] (ab13583)Western blot - Huntingtin antibody [1A771] (ab13583) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May play a role in microtubule-mediated transport or vesicle function.

Tissue specificity

Expressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Involvement in disease

Defects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).

Sequence similarities

Belongs to the huntingtin family.
Contains 10 HEAT repeats.

Domain

The N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.

Post-translational
modifications

Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.

Cellular localization

Cytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.

Target information above from: UniProt accessionP42858 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AI256365 antibody
  • C430023I11Rik antibody
  • HD antibody
  • HD protein antibody
  • HD_HUMAN antibody
  • HDH antibody
  • HTT antibody
  • Huntingtin antibody
  • HUNTINGTON CHOREA antibody
  • Huntington disease protein antibody
  • Huntington's disease protein homolog antibody
  • IT 15 antibody
  • IT15 antibody
  • OTTMUSP00000026909 antibody
  • ZHD antibody
see all

Anti-Huntingtin antibody [1A771] images:

  Western blot - Huntingtin antibody [1A771] (ab13583)

Western blot - Huntingtin antibody [1A771] (ab13583)



Predicted band size : 377 kDa


Western blot analysis of Huntington Disease protein in different lymphoblast cell line extracts (Lanes 1-4) (CAG repeats ~65 and ~20) using Abcam's anti-huntingtin antibody (Panel A), and an antibody from another source (Panel B).

References for Anti-Huntingtin antibody [1A771] (ab13583)

ab13583 has not yet been referenced specifically in any publications.

Publishing research using ab13583? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"