Overview

  • Product nameAnti-Huntingtin antibody [EP867Y]See all Huntingtin primary antibodies ...
  • Description
    Rabbit monoclonal [EP867Y] to Huntingtin
  • Tested applicationsWB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues specific to the apopain cleavage site of human Huntingtin.

  • Positive control
    • SKNSH cell line (IF/ICC)
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab45169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 348 kDa.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMay play a role in microtubule-mediated transport or vesicle function.
    • Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
    • Involvement in diseaseDefects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
    • Sequence similaritiesBelongs to the huntingtin family.
      Contains 10 HEAT repeats.
    • DomainThe N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
    • Post-translational
      modifications
      Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
      Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
    • Cellular localizationCytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI256365 antibody
      • C430023I11Rik antibody
      • HD antibody
      • HD protein antibody
      • HD_HUMAN antibody
      • HDH antibody
      • HTT antibody
      • Huntingtin antibody
      • HUNTINGTON CHOREA antibody
      • Huntington disease protein antibody
      • Huntington's disease protein homolog antibody
      • IT 15 antibody
      • IT15 antibody
      • OTTMUSP00000026909 antibody
      • ZHD antibody
      see all

    Anti-Huntingtin antibody [EP867Y] images

    • Anti-Huntingtin antibody [EP867Y] (ab45169) at 1/10000 dilution + SH-SY-5Y cell lysate

      Predicted band size : 348 kDa
      Observed band size : 300 kDa (why is the actual band size different from the predicted?)
    • Ab45169 staining human Huntingtin in human brain tissue by immunohistochemistry using paraffin embedded tissue.Ab45169 staining human Huntingtin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
    • Overlay histogram showing SH-SY5Y cells stained with ab45169 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45169, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    • ICC/IF image of ab45169 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45169, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Huntingtin antibody [EP867Y] (ab45169)

    This product has been referenced in:
    • Czeredys M  et al. Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease. Front Mol Neurosci 6:42 (2013). WB ; Human . Read more (PubMed: 24324398) »
    • Clemen CS  et al. Strumpellin is a novel valosin-containing protein binding partner linking hereditary spastic paraplegia to protein aggregation diseases. Brain 133:2920-41 (2010). Read more (PubMed: 20833645) »

    See all 3 Publications for this product

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