Anti-Huntingtin antibody [EPR5526] (ab109115)
- Product nameAnti-Huntingtin antibody [EPR5526]See all Huntingtin primary antibodies ...
- DescriptionRabbit monoclonal [EPR5526] to Huntingtin
- Tested applicationsWB, IHC-P, ICC more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to residues in the N terminus of Human Huntingtin.
- Positive control
- WB: SH-SY5Y, HeLa and U87-MG cell lysates IHC-P: Human brain tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.
- General notesProduced under U.S. Patent No. 5,675,063
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5526
- Research Areas
Our Abpromise guarantee covers the use of ab109115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/10000 - 1/50000. Detects a band of approximately 348 kDa (predicted molecular weight: 348 kDa).|
|IHC-P||IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.|
|ICC||ICC: 1/50 - 1/100.|
- FunctionMay play a role in microtubule-mediated transport or vesicle function.
- Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
- Involvement in diseaseDefects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
- Sequence similaritiesBelongs to the huntingtin family.
Contains 10 HEAT repeats.
- DomainThe N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
modificationsCleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
- Cellular localizationCytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
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Anti-Huntingtin antibody [EPR5526] images
All lanes : Anti-Huntingtin antibody [EPR5526] (ab109115) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 348 kDa
Observed band size : 348 kDa
ab109115, at 1/100, staining Huntingtin in paraffin embedded Human brain tissue by Immunohistochemistry.
ab109115 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109115 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Huntingtin antibody [EPR5526] (ab109115)
ab109115 has not yet been referenced specifically in any publications.