Overview

  • Product nameAnti-Huntingtin antibody
    See all Huntingtin primary antibodies
  • Description
    Mouse polyclonal to Huntingtin
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Fruit fly (Drosophila melanogaster)
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 1156-1175 of Fruit fly (Drosophila melanogaster) Huntingtin. Target sequence:

    DNALSSQRQQQRRPNDAGTC

    .

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab43554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 395 kDa.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in microtubule-mediated transport or vesicle function.
    • Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
    • Involvement in diseaseDefects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
    • Sequence similaritiesBelongs to the huntingtin family.
      Contains 10 HEAT repeats.
    • DomainThe N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
    • Post-translational
      modifications
      Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
      Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
    • Cellular localizationCytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
    • Information by UniProt
    • Database links
      • Alternative names
        • AI256365 antibody
        • C430023I11Rik antibody
        • HD antibody
        • HD protein antibody
        • HD_HUMAN antibody
        • HDH antibody
        • HTT antibody
        • Huntingtin antibody
        • HUNTINGTON CHOREA antibody
        • Huntington disease protein antibody
        • Huntington's disease protein homolog antibody
        • IT 15 antibody
        • IT15 antibody
        • OTTMUSP00000026909 antibody
        • ZHD antibody
        see all

      Anti-Huntingtin antibody images

      • All lanes : Anti-Huntingtin antibody (ab43554) at 1/1000 dilution

        Lane 1 : Total protein extract from E coli with 50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen
        Lane 2 : Total protein extract from E coli with 50ng to 500ng of the antigen (recombinant fusion protein containing a His tag)

        Secondary
        Rabbit anti-mouse IgG + IgM, HRP conjugated at 1/5000 dilution

        Predicted band size : 395 kDa

      References for Anti-Huntingtin antibody (ab43554)

      ab43554 has not yet been referenced specifically in any publications.

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