This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Huntingtin (phospho S421) antibody (ab2174)

Overview

  • Product nameAnti-Huntingtin (phospho S421) antibodySee all Huntingtin primary antibodies ...
  • Description
    Rabbit polyclonal to Huntingtin (phospho S421)

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • SpecificityDetects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
  • Species reactivity

    Predicted to work with: Human, Pig
  • Immunogen

    Synthetic peptide: RSRSGpSIVE, corresponding to amino acids 416-424 of Human Huntingtin

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Notes
ELISA Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide .
IHC-Fr 1/250.

Target

  • FunctionMay play a role in microtubule-mediated transport or vesicle function.
  • Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
  • Involvement in diseaseDefects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
  • Sequence similaritiesBelongs to the huntingtin family.
    Contains 10 HEAT repeats.
  • DomainThe N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
  • Post-translational
    modifications
    Cleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
    Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
  • Cellular localizationCytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.
  • Target information above from: UniProt accession P42858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AI256365 antibody
    • C430023I11Rik antibody
    • HD antibody
    • HD protein antibody
    • HD_HUMAN antibody
    • HDH antibody
    • HTT antibody
    • Huntingtin antibody
    • HUNTINGTON CHOREA antibody
    • Huntington disease protein antibody
    • Huntington's disease protein homolog antibody
    • IT 15 antibody
    • IT15 antibody
    • OTTMUSP00000026909 antibody
    • ZHD antibody
    see all

Anti-Huntingtin (phospho S421) antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate

    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,130 kDa. We are unsure as to the identity of these extra bands.
  • ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).

References for Anti-Huntingtin (phospho S421) antibody (ab2174)

ab2174 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Permeabilization Yes - 1% Triton X-100 in TBS
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Marian Hruska-Plochan

Verified customer

Submitted Oct 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"