Anti-Huntingtin (phospho S421) antibody (ab2174)

Overview

• Product nameAnti-Huntingtin (phospho S421) antibodySee all Huntingtin primary antibodies ...
• Description
  Rabbit polyclonal to Huntingtin (phospho S421)

  This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

• SpecificityDetects a 190kDa band in PC-3 cells, which may correspond to Huntingtin. Stains neuronal cytoplasm in immunohistochemistry (the expected localisation). The antibody has not been conclusively tested for specificity for phospho S421 Huntingtin.
• Species reactivity
  
  Predicted to work with: Human, Pig
• Immunogen

  Synthetic peptide: RSRSG_pSIVE, corresponding to amino acids 416-424 of Human Huntingtin

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% Sodium Azide, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Huntington's disease

Applications

Target

• FunctionMay play a role in microtubule-mediated transport or vesicle function.
• Tissue specificityExpressed in the brain cortex (at protein level). Widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.
• Involvement in diseaseDefects in HTT are the cause of Huntington disease (HD) [MIM:143100]. HD is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia. Onset of the disease occurs usually in the third or fourth decade of life and symptoms progressively worsen leading to death in 10 to 20 years. Onset and clinical course depend on the degree of poly-Gln repeat expansion, longer expansions resulting in earlier onset and more severe clinical manifestations. HD affects 1 in 10,000 individuals of European origin. Neuropathology of Huntington disease displays a distinctive pattern with loss of neurons, especially in the caudate and putamen (striatum).
• Sequence similaritiesBelongs to the huntingtin family.
  Contains 10 HEAT repeats.
• DomainThe N-terminal Gln-rich and Pro-rich domain has great conformational flexibility and is likely to exist in a fluctuating equilibrium of alpha-helical, random coil, and extended conformations.
• Post-translational
  modificationsCleaved by apopain downstream of the polyglutamine stretch. The resulting N-terminal fragment is cytotoxic and provokes apoptosis.
  Forms with expanded polyglutamine expansion are specifically ubiquitinated by SYVN1, which promotes their proteasomal degradation.
• Cellular localizationCytoplasm. Nucleus. The mutant Huntingtin protein colocalizes with AKAP8L in the nuclear matrix of Huntington's disease neurons.

Target information above from: UniProt accession P42858 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3064 Human
  • Omim: 143100 Human
  • Omim: 613004 Human
  • SwissProt: P42858 Human
  • Unigene: 518450 Human

• Alternative names
  AI256365 antibodyC430023I11Rik antibodyHD antibody

  HD protein antibodyHD_HUMAN antibodyHDH antibodyHTT antibodyHuntingtin antibodyHUNTINGTON CHOREA antibodyHuntington disease protein antibodyHuntington's disease protein homolog antibodyIT 15 antibodyIT15 antibodyOTTMUSP00000026909 antibodyZHD antibody

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Anti-Huntingtin (phospho S421) antibody images

• 

  Western blot - Huntingtin (phospho S421) antibody (ab2174)
  Anti-Huntingtin (phospho S421) antibody (ab2174) at 1/1000 dilution + human PC-3 cell lysate
  
  Observed band size : 190 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 120 kDa,130 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Huntingtin (phospho S421) antibody (ab2174)
  ab2174 was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Detection was with AEC (pink), nuclear counterstaining with Haemotoxylin (blue).