Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody (ab176992)
Key features and details
- Rabbit polyclonal to Hydrogen Potassium ATPase Beta/ATP4B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody
See all Hydrogen Potassium ATPase Beta/ATP4B primary antibodies -
Description
Rabbit polyclonal to Hydrogen Potassium ATPase Beta/ATP4B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Hydrogen Potassium ATPase Beta/ATP4B aa 167-290.
Sequence:DPNF GFEEGKPCFI IKMNRIVKFL PSNGSAPRVD CAFLDQPREL GQPLQVKYYP PNGTFSLHYF PYYGKKAQPH YSNPLVAAKL LNIPRNAEVA IVCKVMAEHV TFNNPHDPYE GKVEFKLKIE
Database link: P51164 -
Positive control
- Mouse heart lysate, Human colon cancer tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:200 ul sterile water -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/200 - 1/1000. Predicted molecular weight: 33 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/200 - 1/1000. Predicted molecular weight: 33 kDa. |
Target
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Function
Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Domain
The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 496 Human
- Entrez Gene: 11945 Mouse
- Omim: 137217 Human
- SwissProt: P51164 Human
- SwissProt: P50992 Mouse
- Unigene: 434202 Human
- Unigene: 154039 Mouse
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Alternative names
- Gastric H(+)/K(+) ATPase subunit beta antibody
- ATP4B antibody
- ATP4B_HUMAN antibody
see all
Images
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Immunohistochemical staining of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Hydrogen Potassium ATPase Beta/ATP4B with ab176992 at 1/100 dilution showing membrane staining
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Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody (ab176992) at 1/500 dilution + Mouse heart lysate
Predicted band size: 33 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab176992 has been referenced in 2 publications.
- Ali II et al. Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis. Biology (Basel) 10:N/A (2021). PubMed: 34439938
- Han L et al. A case report of endoscopic submucosal dissection for a new subtype of gastric adenoma: mixed fundic and pyloric mucosa type. Transl Cancer Res 10:3053-3058 (2021). PubMed: 35116613