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Overview

  • Product nameI-309 (CCL1) Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 4 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 79.9 70% - 103%
    Serum 106.9 97% - 116%
    Plasma 97.65 88% - 110%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionCytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P22362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        BF534335C C motif chemokine 1C-C motif chemokine 1
        Ccl1CCL1_HUMANCCR8 ligandChemokine CC Motif Ligand 1Inflammatory cytokine I-309 P500RP23-446K18.2SCYA1SISeSmall inducible cytokine A1 Small-inducible cytokine A1T lymphocyte secreted protein I-309T lymphocyte-secreted protein I-309TCA3
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100536 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      I-309 (CCL1) Human ELISA Kit images

      • Typical standard curve
        Typical standard curve

        Representative standard curve using ab100536

      References for I-309 (CCL1) Human ELISA Kit (ab100536)

      ab100536 has not yet been referenced specifically in any publications.

      Publishing research using ab100536 ? Please let us know so that we can cite the reference in this datasheet

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      I have confirmed that the customer followed all of the instructions as suggested for reconstitution, storage and handling of the standard. The customer reconstituted the second vial of standard for attempt #2. The customer has also followed all dilutions ...

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      The customer who has received this kit has reported a problem with this kit, after trying the assay twice and seeing poor standard curve results. Please see data attached. Please let me know if you need any further information from this customer.

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