Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-IARS2 antibody
See all IARS2 products (2) ...
Description
Rabbit polyclonal to IARS2
Specificity
ab66012 detects endogenous levels of total IARS2 protein.
Tested applications
ELISA, IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from an internal sequence of human IARS2.
Positive control
IHC-P: Human breast carcinoma tissue ICC/IF: HepG2 cells WB: Extracts from RAW264.7 cells and COS7 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab66012 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
WB: 1/500 - 1/1000.Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
Target
Relevance
IARS2 belongs to the class-I aminoacyl-tRNA synthetase family.
Cellular localization
Mitochondrion matrix
Alternative names
- C79125 antibody
- MGC63429 antibody
- 2010002H18Rik antibody
see all
Database links
- Entrez Gene: 55699 Human
- Entrez Gene: 381314 Mouse
- SwissProt: Q9NSE4 Human
- SwissProt: Q8BIJ6 Mouse
- Unigene: 262823 Human
Anti-IARS2 antibody images:
Immunocytochemistry/ Immunofluorescence - IARS2 antibody (ab66012)
ab66012 at 1/500 dilution staining IARS2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IARS2 antibody (ab66012)
ab66012, at a 1/50 dilution, staining IARS2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
Western blot - IARS2 antibody (ab66012)
All lanes : Anti-IARS2 antibody (ab66012) at 1/500 dilution
Lane 1 : RAW264.7 cell extracts
Lane 2 : COS7 cell extracts
Lane 3 : COS7 cell extracts with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 114 kDa
Observed band size : 114 kDa
References for Anti-IARS2 antibody (ab66012)
ab66012 has not yet been referenced specifically in any publications.
Publishing research using ab66012? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"