Anti-ICA69 antibody (ab154391)
Key features and details
- Rabbit polyclonal to ICA69
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ICA69 antibody -
Description
Rabbit polyclonal to ICA69 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human ICA69 aa 1-223.
Database link: Q05084 -
Positive control
- A549 whole cell lysate; Human A2780-CP70 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in neurotransmitter secretion. -
Tissue specificity
Expressed abundantly in pancreas, heart and brain with low levels of expression in lung, kidney, liver and thyroid. -
Sequence similarities
Contains 1 AH domain. -
Cellular localization
Cytoplasm > cytosol. Golgi apparatus membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Predominantly cytosolic. Also exists as a membrane-bound form which has been found associated with synaptic vesicles and also with the Golgi complex and immature secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 535346 Cow
- Entrez Gene: 3382 Human
- Entrez Gene: 15893 Mouse
- Entrez Gene: 81024 Rat
- Entrez Gene: 414587 Xenopus laevis
- Entrez Gene: 549616 Xenopus tropicalis
- Omim: 147625 Human
- SwissProt: Q05084 Human
see all -
Alternative names
- 69 kDa islet cell autoantigen antibody
- Diabetes mellitus type I autoantigen antibody
- ICA 1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154391 has been referenced in 1 publication.
- Kong C et al. ICA69 aggravates ferroptosis causing septic cardiac dysfunction via STING trafficking. Cell Death Discov 8:187 (2022). PubMed: 35397620