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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Overview
Product name
Anti-ICA1 antibody
See all ICA1 products (3) ...
Description
Rabbit polyclonal to ICA1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 432-481 (DQNMKDLQAS LQEPAKAASD LTAWFSLFAD LDPLSNPDAV GKTDKEHELL) of Human ICA1 (NP_071682).
DQNMKDLQAS LQEPAKAASD LTAWFSLFAD LDPLSNPDAV GKTDKEHELL
Positive control
Fetal muscle lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Immunology >> Immune System Diseases >> Autoimmune
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
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Western blot - ICA1 antibody (ab81499)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
ELISA titre using peptide based assay 1/312500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play a role in neurotransmitter secretion.
Tissue specificity
Expressed abundantly in pancreas, heart and brain with low levels of expression in lung, kidney, liver and thyroid.
Sequence similarities
Contains 1 AH domain.
Cellular localization
Cytoplasm > cytosol. Golgi apparatus membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Predominantly cytosolic. Also exists as a membrane-bound form which has been found associated with synaptic vesicles and also with the Golgi complex and immature secretory granules.
Target information above from: UniProt accessionQ05084
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 69 kDa islet cell autoantigen antibody
- Diabetes mellitus type I autoantigen antibody
- ICA 1 antibody
see all
Database links
- Entrez Gene: 3382 Human
- Entrez Gene: 15893 Mouse
- Entrez Gene: 81024 Rat
- Omim: 147625 Human
- SwissProt: Q05084 Human
- SwissProt: P97411 Mouse
- SwissProt: Q63054 Rat
- SwissProt: Q6AZ05 Rat
see all
Anti-ICA1 antibody images:
Western blot - ICA1 antibody (ab81499)
Anti-ICA1 antibody (ab81499) at 1 µg/ml + fetal muscle lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Gel concentration 12%
References for Anti-ICA1 antibody (ab81499)
ab81499 has not yet been referenced specifically in any publications.
Publishing research using ab81499? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"