Anti-ICAM1 antibody [1A29] (ab171123)
Key features and details
- Mouse monoclonal [1A29] to ICAM1
- Suitable for: ICC, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-ICAM1 antibody [1A29]
See all ICAM1 primary antibodies -
Description
Mouse monoclonal [1A29] to ICAM1 -
Host species
Mouse -
Tested applications
Suitable for: ICC, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Rat ICAM1. Rat lymph node stroma - Native protein
Database link: Q00238 -
Positive control
- IHC-P: Mouse kidney, lung and spleen tissues. WB: Mouse kidney, lung and spleen tissue lysate; Rat heart tissue lysate; A549 cell lysate. Flow Cyt: C6, Raji and Ramos cells. ICC: A439 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS
Preservative negative and carrier free. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1A29 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/250.
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WB |
1/250. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
1/20.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC
1/250. |
WB
1/250. Predicted molecular weight: 57 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
1/20. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3383 Human
- Entrez Gene: 15894 Mouse
- Entrez Gene: 25464 Rat
- Omim: 147840 Human
- SwissProt: P05362 Human
- SwissProt: P13597 Mouse
- SwissProt: Q00238 Rat
- Unigene: 643447 Human
see all -
Alternative names
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
Images
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All lanes : Anti-ICAM1 antibody [1A29] (ab171123) at 1/250 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse kidney lysate
Lane 4 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Western blot analysis was performed on whole cell extracts. The blots were probed with ab171123 (1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate. A 56 kDa band corresponding to ICAM-1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel , XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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Immunohistochemical analysis of deparaffinized mouse kidney tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.
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Flow cytometry analysis of ICAM1 in C6 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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Immunocytochemical analysis of A439 cells staining ICAM1 with ab171123 at 1ug/ml.
Cells were fixed with 4% paraformaldehyde for 15 minutes, permeablilized with 0.25% Trition X-100 for 10 minutes and blocked with 5% BSA for 1 hour at room temparature. Cells were incubated with primary antibody at room temperature for 3 hours. Goat Anti-Mouse IgG (H&L) (Alexa Fluor® 488) was used as a secondary antibody.
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Flow cytometry analysis of ICAM1 in Raji cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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Flow cytometry analysis of ICAM1 in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ICAM1 monoclonal antibody (ab171123) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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Immunohistochemical analysis of deparaffinized mouse spleen tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.
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Immunohistochemical analysis of deparaffinized mouse lung tissue, labeling ICAM1 with ab171123 at 1/100 dilution (left image) compared with a negative control without primary antibody (right image). Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab171123 has been referenced in 61 publications.
- Yu S et al. Melatonin prevents experimental central serous chorioretinopathy in rats. J Pineal Res 73:e12802 (2022). PubMed: 35436360
- Weng TH et al. Anti-Inflammatory Effects of GM1 Ganglioside on Endotoxin-Induced Uveitis in Rats. Biomolecules 12:N/A (2022). PubMed: 35625654
- Xiong J et al. CircRNA mmu_circ_0000021 regulates microvascular function via the miR-143-3p/NPY axis and intracellular calcium following ischemia/reperfusion injury. Cell Death Discov 8:315 (2022). PubMed: 35821018
- Yuan Q et al. Neobavaisoflavone ameliorates LPS-induced RAW264.7 cell inflammations by suppressing the activation of NF-κB and MAPKs signaling pathways. Iran J Basic Med Sci 25:1021-1027 (2022). PubMed: 36159335
- Chen Y et al. The Mechanism of Alisol B23 Acetate Inhibiting Lung Cancer: Targeted Regulation of CD11b/CD18 to Influence Macrophage Polarization. Drug Des Devel Ther 16:3677-3689 (2022). PubMed: 36277599