ICAM1 Human ELISA Kit (ab100640)
- Product nameICAM1 Human ELISA KitSee all ICAM1 kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range24.58 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.37 89% - 107% Serum 97.48 88% - 106% Plasma 98.67 90% - 107%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
ab100640 ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ICAM1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody sICAM-1: biotinylated anti-human sICAM-1 2 vials HRP-Streptavidin Concentrate 340x concentrated HRP-conjugated streptavidin 1 x 200µl ICAM 1 Microplate (12 strips x 8 wells) coated with anti-human ICAM-1 1 unit ICAM 1 Standard Human (recombinant) 2 vials Stop Solution 1 x 25ml TMB One-Step Substrate Reagent 1 x 12ml
- Research Areas
- BB2CD 54 CD54
- CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195MyD10P3.58 Surface antigen of activated B cells
Our Abpromise guarantee covers the use of ab100640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for ICAM1 Human ELISA Kit (ab100640)
ab100640 has not yet been referenced specifically in any publications.