You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameICAM1 Human ELISA KitSee all ICAM1 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 30 pg/ml
  • Range
    24.58 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.37 89% - 107%
    Serum 97.48 88% - 106%
    Plasma 98.67 90% - 107%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100640 ICAM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ICAM1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details
  • PlatformMicroplate

Properties

  • RelevanceICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • Cellular localizationMembrane
    • Alternative names
        BB2CD 54 CD54
        CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195MyD10P3.58 Surface antigen of activated B cells
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100640 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      ICAM1 Human ELISA Kit images

      • ELISA - ICAM1 Human ELISA Kit (ab100640)
        ELISA - ICAM1 Human ELISA Kit (ab100640)

      Protocols

      References for ICAM1 Human ELISA Kit (ab100640)

      ab100640 has not yet been referenced specifically in any publications.

      Publishing research using ab100640 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100640 Submit a Question for ab100640

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

      10
      Votes: Highest First

      Hi Abcam,

      Could you please advise if these kits can be used to test citrate plasma?

      Thanks for your help!

      Kind regards,

      Thank you for contacting us.

      All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

      I hope this information has been of help. If your require any further information please do not he...

      Read More

      Hi
      is the antibody in this kit monoclonal or polyclonal. it is not clear in the information.
      ICAM1 Human ELISA Kit - 1 x 96 Well Plate (ab100640)

      Thank you for your enquiry and your interest in our products.

      The capture antibody of the ab100640 (ICAM1 Human ELISA Kit - 1 x 96) kit is a monoclonal mouse IgG1.

      If you need any further assistance in the future, please do not hesitat...

      Read More

      What serum dilutions should be used with these 4 kits? uses human serum samples

      Thank you for contacting us. Here is the requested information: ab46087: Normal values for TNFa in such samples are negatives or very low, so we usually recommend to assay the human serum samples neat with Standard buffer, human serum to dilute the ...

      Read More

      Yes the ICAM in human serum/plasma is in the 200-300 ng/ml range but the high end of the curve for the Abcam kit (AB100640) is 2000pg/ml or 2 ng/ml. So do we need a 200-300 fold dilution before assaying. That was the question we posed

      Please accept my apologies for the miscommunication in the previous email about ab100640, the ICAM1 Human ELISA Kit. Yes, you would need to do a dilution of your samples, so that they would be within the detection range of the kit. Please let me know if...

      Read More

      I purchased the following kits and there are issues with the sample range: ab100640, ICAM1 Human ELISA Kit - 1 x 96 - Normal human sample range is from 0.2-1pg/ml. However the kits range is 24.58 pg/ml - 6000 pg/ml.  Will the kit recognises samples as low...

      Read More

      Thank you for contacting Abcam. I have received a reply from the lab about ab100640, the ICAM1 Human ELISA Kit. Based on testing and the reported literature, normal levels of soluble ICAM in serum look to be in the low-to-mid ng/ml levels and not low pg/m...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"