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ICAM1 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47361)

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Overview

  • Product nameICAM1 Human Elipair Kit (Reagents for 10 x 96 Well Plates)See all ICAM1 kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 0.1 ng/ml
  • Range
    0.25 ng/ml - 8 ng/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Intended Use

    This Eli-pair set is intended to measure human ICAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Principle of Method

    The ICAM-1 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human ICAM1

  • Tested applicationsELISA more details

Properties

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P05362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        BB2CD54ICAM-1
        ICAM1ICAM1_HUMANIntercellular adhesion molecule 1Major group rhinovirus receptor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab47361 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA
    • Application notesELISA: Use at an assay dependent dilution.


      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.

      • ICAM1 Human Elipair Kit (Reagents for 10 x 96 Well Plates) images

      • Eli-pair - Human ICAM1 Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47361)
        Eli-pair - Human ICAM1 Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47361)
        ICAM-1 Standard curve ranging from 0.25 to 8 ng/mL

      References for ICAM1 Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47361)

      ab47361 has not yet been referenced specifically in any publications.

      Publishing research using ab47361 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab47361 Submit a Question for ab47361

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      Dear Sir,



      Thank you for quick answer.



      ICAM1 ab 47361 lot: GR55579-2

      VCAM1 ab47355 lot: GR55578-1



      Please ask your laboratory about precise protocol.



      How ...

      Read More

      Thanks for your enquiry.

      Please find below additional info required for ab47355 and ab47361


      1) PBS is prepared as followed : 0.137mM NaCl; 1.47 KH2PO4; 8.1mM Na2PO4, 2H2O

      2) Samples should be diluted : 1/100 to 1/200 ...

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      I am writing you in a favor of Eli Pair kit (ab47361). We have done all procedures as you have recommended, but standards absorbances were to low. The highest absorbance was 0,1 for solution of 8ng/ml, and absorbances for patients samples were in the rang...

      Read More

      Thank you for bringing this to our attention. The laboratory that produces this set of ELISA reagents has not reported any issues with any batches of the kit. If you have followed the protocol, then I am not sure what the problem is.Your results demonstra...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"