ICAM1 Monkey ELISA Kit (with color giving dyes) (ab119527)
Overview
- Product nameICAM1 Monkey ELISA Kit (with color giving dyes)See all ICAM1 kits ...
- Precision
Intra-assay Sample n Mean SD CV% Overall < 5% Inter-assay Sample n Mean SD CV% Overall < 10% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity1.1 U/ml
- Range6.3 U/ml - 100 U/ml
- Recovery
95 %
- Species reactivityReacts with: Monkey
- Product overview
ab119527 ICAM1 Monkey ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sICAM-1. ab119527 is for research use only and is not for diagnostic or therapeutic procedures.
Intercellular Adhesion Molecule-1 (ICAM1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95.
ICAM1 is a single-chain glycoprotein with a polypeptide core that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
The role of ICAM1 as a disease marker has been demonstrated for a number of different indications and pathological situations.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 2 units Adhesive Films 2 units Assay Buffer Concentrate (20x) 1 x 5ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml HRP-Conjugate anti-monkey sICAM1 monoclonal antibody 1 x 100µl Monkey sICAM1 Standard 2 x 500µl Monoclonal anti-monkey sICAM1-coated microwell plate 1 unit Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml - Research Areas
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Alternative names
- BB2CD 54 CD54
- CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195MyD10P3.58 Surface antigen of activated B cells
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Applications
Our Abpromise guarantee covers the use of ab119527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
ICAM1 Monkey ELISA Kit (with color giving dyes) images
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Sandwich ELISA - ICAM1 Monkey ELISA Kit (with color giving dyes) (ab119527)Representative standard curve using ab119527.
Protocols
References for ICAM1 Monkey ELISA Kit (with color giving dyes) (ab119527)
ab119527 has not yet been referenced specifically in any publications.
