ICAM1 Mouse ELISA Kit (ab100688)
- Product nameICAM1 Mouse ELISA KitSee all ICAM1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 20 pg/ml
- Range24.69 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.54 84% - 103% Serum 94.56 84% - 103% Plasma 115.2 97% - 127%
- Species reactivityReacts with: Mouse
- Product overview
ab100688 Mouse ICAM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse ICAM1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium) diluent. 1 x 15ml Detection Antibody ICAM1: biotinylated anti-mouse ICAM1 2 vials ICAM1 Microplate (12 strips x 8 wells) coated with anti-mouse ICAM1 1 unit ICAM1 Standard Mouse (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- BB2CD 54 CD54
- CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195MyD10P3.58 Surface antigen of activated B cells
Our Abpromise guarantee covers the use of ab100688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for ICAM1 Mouse ELISA Kit (ab100688)
ab100688 has not yet been referenced specifically in any publications.