ICAM1 Rat ELISA Kit (ab100763)
- Product nameICAM1 Rat ELISA KitSee all ICAM1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 20 pg/ml
- Range28.81 pg/ml - 7000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 98.54 89% - 107% Serum 89.42 80% - 101% Plasma 85.54 76% - 95%
- Species reactivityReacts with: Rat
- Product overview
ab100763 Rat ICAM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat ICAM1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A . diluent buffer For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml Detection Antibody ICAM1 : biotinylated anti-rat ICAM1 2 vials ICAM1 Microplate (12 strips x 8 wells) coated with anti-rat ICAM1 1 unit ICAM1 Standard Rat (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- BB2CD 54 CD54
- CD54 antigen Cell surface glycoprotein P3.58 Human rhinovirus receptor ICAM 1Intercellular adhesion molecule 1 Major group rhinovirus receptor MALA2 MGC6195MyD10P3.58 Surface antigen of activated B cells
Our Abpromise guarantee covers the use of ab100763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for ICAM1 Rat ELISA Kit (ab100763)
ab100763 has not yet been referenced specifically in any publications.