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We're considering the antibody ab20 for use in functional studies, but we wanted to make sure that it was not in a buffer containing azide. Would you please let me know whether it does contain azide? |
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ANSWER: |
This antibody does not contain azide |
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Hello, That data sheets says "raised in Balb/c" - sorry for my ignorance. Is this rat or mouse? Thank you in advance. |
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ANSWER: |
Balb/c is a mouse strain |
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Are all antibodies 2952 - 2955 of the IgG1 subclass? This is important for us to chose the right secondary antibody - we are considering IgG1 PerCP from Becton Dickinson. Are you aware of a different red-shifted secondary antibody one could use with 488 laser excitation? Thanks a lot, kind regards, Ulf Steller Janssen Research Foundation Belgium |
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ANSWER: |
Yes, all abs 2952-2955 are IgG1. Abcam has a FITC conjugated sheep anti-mouse IgG secondary, product code ab11-500, which may be suitable for your needs. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
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