ICAM1 antibody [15.2] (ab20)

Product Name 

ICAM1 antibody [15.2]

Product type 

Primary antibodies

Description 

Mouse monoclonal [15.2] to ICAM1

Immunogen 

Human monocytes.

Reacts with 
(species key)

Hu

Specificity 

Blocks binding of LFA-1 & infected RBCs to ICAM1.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.

IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
WB: Use at a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.

Is unsuitable for IHC-P.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tonsil

Cellular localization 

Type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion

Relevance 

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

15.2

Myeloma 

Sp2/0

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Phosphate buffered saline

Form 

Liquid

Concentration 

Batch dependent within range: 1.10 - 1.10 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for ICAM1 antibody [15.2] images, references, products related to ab20 and other tools.

ICAM1 antibody [15.2] images:

References for ICAM1 antibody [15.2] (ab20)

  This product has been used in: (two most recent references)

Stanley P et al. The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain. Biochem J 351:79-86 (2000). PubMed: 10998349

Dransfield I et al. Interaction of leukocyte integrins with ligand is necessary but not sufficient for function. J Cell Biol 116:1527-35 (1992). PubMed: 1541641
If you publish research using ab20 please let us know so that we can cite the reference on this datasheet.

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