![Western blot - ICAM1 antibody [15.2] (ab20) image](#ICAM1-Primary-antibodies-ab20-2.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM1 antibody [15.2]
See all ICAM1 products (46) ...
Description
Mouse monoclonal [15.2] to ICAM1
Specificity
Blocks binding of LFA-1 & infected RBCs to ICAM1.
Tested applications
WB, Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Human monocytes.
Positive control
Tonsil
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
15.2
Myeloma
Sp2/0
Isotype
IgG1
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - ICAM1 antibody [15.2] (ab20)
![Western blot - ICAM1 antibody [15.2] (ab20) image](/ps/datasheet/images/0/ab20/ICAM1-Primary-antibodies-ab20-2.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlPredicted molecular weight: 58 kDa.
Flow Cyt: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
Application notes
Is unsuitable for or IHC-P.
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Anti-ICAM1 antibody [15.2] images:
Western blot - ICAM1 antibody [15.2] (ab20)
All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
References for Anti-ICAM1 antibody [15.2] (ab20)
This product has been referenced in:
- Allen CEet al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr; Human.Read more (PubMed: 20220088) »
- Stanley Pet al. The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain. Biochem J 351:79-86 (2000).Read more (PubMed: 10998349) »
See all 3 publications for this product
Publishing research using ab20? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"