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Read our guarantee »Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Anti-ICAM1 antibody [15.2]
See all ICAM1 products (46) ...
Mouse monoclonal [15.2] to ICAM1
Blocks binding of LFA-1 & infected RBCs to ICAM1.
WB, Flow Cyt, IHC-Fr, IPmore details
Reacts with
Human
Human monocytes.
Tonsil
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS
Concentration information loading...
Protein A purified
Monoclonal
15.2
Sp2/0
IgG1
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Western blot - ICAM1 antibody [15.2] (ab20)
(enlarge)
Our Abpromise guarantee covers the use of ab20 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlPredicted molecular weight: 58 kDa.
Flow Cyt: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
Is unsuitable for or IHC-P.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - ICAM1 antibody [15.2] (ab20)
![Western blot - ICAM1 antibody [15.2] (ab20)](/ps/datasheet/images/0/ab20/ICAM1-Primary-antibodies-ab20-2.jpg)
All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab20? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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![Western blot - ICAM1 antibody [15.2] (ab20)](/ps/datasheet/images/0/ab20/ICAM1-Primary-antibodies-ab20-2.jpg)
All lanes : Anti-ICAM1 antibody [15.2] (ab20) at 5 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
0
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