Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-ICAM1 antibody [1A29] (Biotin)
See all ICAM1 products (44) ...
Description
Mouse monoclonal [1A29] to ICAM1 (Biotin)
Conjugation
Biotin
Specificity
ab33884 recognises the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a 90kD adhesion molecule belonging to the immunoglobulin superfamily.
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Rat
Immunogen
Tissue / cell preparation (Rat)- Ax cells (a HEV derived cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
1A29
Myeloma
PAI
Isotype
IgG1
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
Application notes
Flow Cyt: Use Neat - 1/5. Use 10µl of this dilution for 106 cells in 100µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 25464 Rat
- SwissProt: Q00238 Rat
- Unigene: 12 Rat
References for Anti-ICAM1 antibody [1A29] (Biotin) (ab33884)
ab33884 has not yet been referenced specifically in any publications.
Publishing research using ab33884? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"