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Anti-ICAM1 antibody [1A29] - Low endotoxin, Azide free (ab33894)

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Overview

Product name

Anti-ICAM1 antibody [1A29] - Low endotoxin, Azide free
See all ICAM1 products (44) ...

Description

Mouse monoclonal [1A29] to ICAM1 - Low endotoxin, Azide free

Specificity

Recognises a 90kD adhesion molecule belonging to the immunoglobulin superfamily.

Tested applications

ICC/IF, IHC-P, IHC-Fr, IP, WB, Functional Studies, Flow Cytmore details

Cross reactivity

Reacts with

Rat

Immunogen

Tissue / cell preparation (Rat) -Ax cells (a HEV derived cell line).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None, No stabilisers, Low endotoxin levels -<0.01EU/µg
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab33894 was purified from tissue culture supernatant

Clonality

Monoclonal

Clone number

1A29

Myeloma

PAI

Isotype

IgG1

Research areas

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution.

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    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.

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    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution. ab33894 functionally inhibits homotypic aggregation of PHA blasts.

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    Flow Cyt

     Flow Cyt: 1/25 - 1/50. U...Read more →

    Flow Cyt: 1/25 - 1/50. Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Target

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Sequence similarities

Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

Post-translational
modifications

Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Cellular localization

Membrane.

Target information above from: UniProt accessionP05362 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BB 2 antibody
  • BB2 antibody
  • CD 54 antibody
  • CD54 antibody
  • CD54 antigen antibody
  • Cell surface glycoprotein P3.58 antibody
  • Human rhinovirus receptor antibody
  • ICAM 1 antibody
  • ICAM-1 antibody
  • ICAM1 antibody
  • ICAM1_HUMAN antibody
  • Intercellular adhesion molecule 1 antibody
  • Major group rhinovirus receptor antibody
  • MALA 2 antibody
  • MALA2 antibody
  • MyD 10 antibody
  • MyD10 antibody
  • P3.58 antibody
  • Surface antigen of activated B cells antibody
see all

Database links

References for Anti-ICAM1 antibody [1A29] - Low endotoxin, Azide free (ab33894)

This product has been referenced in:

  • Williams BA  et al. Impact of arteriogenesis in plastic surgery: choke vessel growth proceeds via arteriogenic mechanisms in the rat dorsal island skin flap. Microcirculation 16:235-50 (2009). WB; Rat.Read more (PubMed: 19225983) »
  • Conde-Vancells J  et al. Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res 7:5157-66 (2008). WB; Rat.Read more (PubMed: 19367702) »

See all 2 publications for this product

Publishing research using ab33894? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"