Anti-ICAM1 antibody [6G12] (ab96485)

Overview

  • Product nameAnti-ICAM1 antibody [6G12]See all ICAM1 primary antibodies ...
  • Description
    Mouse monoclonal [6G12] to ICAM1
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human ICAM1 (amino acids 28-480) expressed in E. coli.

  • Positive control
    • ICAM1-hIgGFc transfected HEK293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab96485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 58 kDa.
ELISA 1/10000.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • Intercellular adhesion molecule 1 antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody [6G12] images

  • Anti-ICAM1 antibody [6G12] (ab96485) at 1/500 dilution + ICAM1-hIgGFc transfected HEK293 cell lysate

    Predicted band size : 58 kDa

References for Anti-ICAM1 antibody [6G12] (ab96485)

ab96485 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

Lamento mucho los problemas sufridos en el uso de ab96485. Revisando la documentación enviada, dado que faltan detalles del protocolo, me gustaría simplemente dar alguna pauta por si quisiera el cliente...

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Thank you for your inquiry.

The WB on the datasheet of ab96485 does notshowendogenousICAM1 protein.

This shows overexpressed transgenic ICAM1 that was fused with the Fc part of human IgG.

Therefore the size is correct: Read More

Thank you for the response.

Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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While many of our ICAM1 antibodies have been tested in Western blot on human and are guaranteed to work under the terms of our Abpromise, in this instance I would recommend one of the following products. ab96485:http://www.abcam.com/ICAM1-antibody-6...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"