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Anti-ICAM1 antibody [6G12] (ab96485)

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Overview

  • Product nameAnti-ICAM1 antibody [6G12]See all ICAM1 primary antibodies ...
  • Description
    Mouse monoclonal [6G12] to ICAM1
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human ICAM1 (amino acids 28-480) expressed in E. coli.

  • Positive controlICAM1-hIgGFc transfected HEK293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab96485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/2000. Predicted molecular weight: 58 kDa.
ELISA ELISA: 1/10000.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P05362 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Antigen identified by monoclonal antibody BB2 antibodyBB 2 antibodyBB2 antibody
        CD 54 antibodyCD_antigen=CD54 antibodyCD54 antibodyCell surface glycoprotein P3.58 antibodyHuman rhinovirus receptor antibodyICAM 1 antibodyICAM-1 antibodyICAM1 antibodyICAM1_HUMAN antibodyIntercellular adhesion molecule 1 antibodyintercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibodyMajor group rhinovirus receptor antibodyMALA 2 antibodyMALA2 antibodyMyD 10 antibodyMyD10 antibodyP3.58 antibodySurface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [6G12] images

    • Western blot - ICAM1 antibody [6G12] (ab96485)
      Western blot - ICAM1 antibody [6G12] (ab96485)
      Anti-ICAM1 antibody [6G12] (ab96485) at 1/500 dilution + ICAM1-hIgGFc transfected HEK293 cell lysate

      Predicted band size : 58 kDa

    References for Anti-ICAM1 antibody [6G12] (ab96485)

    ab96485 has not yet been referenced specifically in any publications.

    Publishing research using ab96485 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab96485 Submit a Question for ab96485

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

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    Votes: Highest First

    Estimado
    A través de la presente, y disculpando el tiempo de demora en el envío de este correo (me he encontrado fuera del laboratorio), quiero respaldar los resultados que hemos obtenido con el anticuerpo que usted nos envió. He...

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    Gracias por contactarnos.

    Lamento mucho los problemas sufridos en el uso de ab96485. Revisando la documentación enviada, dado que faltan detalles del protocolo, me gustaría simplemente dar alguna pauta por si quisiera el cliente me...

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    Dear all:



    I hope you are well.



    I have a customer who has a doubt about the product ab96485. In the datasheet, it shows a band of 96 kDa of MW, but it says the molecular weight observed was 58 kDa. May you expl...

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    Thank you for your inquiry.

    The WB on the datasheet of ab96485 does notshowendogenousICAM1 protein.

    This shows overexpressed transgenic ICAM1 that was fused with the Fc part of human IgG.

    Therefore the size is correct:
    <...

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    We have informed that the product ab27536, which was discontinued. We have been received the information given by you, but I contacted with the customer and she told me she need the antibody is able to recognize the ICAM1 protein intra- and extracellular ...

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    Thank you for the response.

    Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore an...

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    A customer in Chile was using ab27536, which we discontinued. The customer was very happy with this product and would like to know if we have a substitute to offer. He was using it in human tissue, western blot. The immunogen was a Recombinant fragment, ...

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    While many of our ICAM1 antibodies have been tested in Western blot on human and are guaranteed to work under the terms of our Abpromise, in this instance I would recommend one of the following products. ab96485:http://www.abcam.com/ICAM1-antibody-6G12...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"