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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM1 antibody (Biotin)
See all ICAM1 products (46) ...
Description
Rabbit polyclonal to ICAM1 (Biotin)
Conjugation
Biotin
Specificity
The antibody reacts with both intact human ICAM-1, CD54 and with soluble human ICAM-1.
Tested applications
Sandwich ELISA, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human, Chimpanzee, Baboon, Cynomolgus Monkey, Rhesus monkey
Immunogen
Full length protein (Human).
Positive control
Fresh skin biopsy (small fresh skin biopsy incubated for 4 hours with TNF (1 ng/ml), IL-1 (100 u/ml) or LPS (1 ìg/ml) in tissue culture medium at 37oC).
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% BSA and 10 mM sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Sandwich ELISA - ICAM1 antibody (Biotin) (ab7815)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab7815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.5 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-111] to ICAM1 (ab2213). (For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab2213 as Capture.)
IHC-Fr: Use at an assay dependent concentration. (Fix with acetone.)
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 3383 Human
- Entrez Gene: 15894 Mouse
- Omim: 147840 Human
- SwissProt: P05362 Human
- SwissProt: P13597 Mouse
- Unigene: 643447 Human
- Unigene: 435508 Mouse
Anti-ICAM1 antibody (Biotin) images:
Sandwich ELISA - ICAM1 antibody (Biotin) (ab7815)
Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
References for Anti-ICAM1 antibody (Biotin) (ab7815)
This product has been referenced in:
- Rohlena Jet al. Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion. Cardiovasc Res 81:187-96 (2009).Read more (PubMed: 18805782) »
- Fransson Jet al. Rapid induction of apoptosis in B-cell lymphoma by functionally isolated human antibodies. Int J Cancer 119:349-58 (2006).Read more (PubMed: 16477633) »
See all 3 publications for this product
Publishing research using ab7815? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"