If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab2213? Please let us know so that we can cite the reference in this datasheet
ab2213 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
ab2213 staining ICAM1 in human lung and appendix tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA, pH 8.0, 20 minutes at 100°C. Samples were then incubated with ab2213 at a 1/25 dilution for 20 minutes at 25°C. The secondary used was a DAB detection system.
Image courtesy of an anonymous Abreview.
ab2213 staining human PBL cells by flow cytometry. Cells were formaldehyde fixed and gated on B-cells. The primary antibody was incubated with the sample for 30 minutes at 25°C. The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L). Both antibodies were used undiluted.
This image is courtesy of an Abreview submitted by Dr Karppiah Muthumani
All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution
Lane 1 : Western Markers.
Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.
Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible non-specific binding).
This image is courtesy of an anonymous Abreview
5
Call 01223 696 000 or contact us