Anti-ICAM1 antibody [MEM-111] (ab2213)

  Sandwich ELISA - ICAM1 antibody [MEM-111] (ab2213)

Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [MEM-111] (ab2213)

ab2213 staining ICAM1 in human lung and appendix tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA, pH 8.0, 20 minutes at 100°C. Samples were then incubated with ab2213 at a 1/25 dilution for 20 minutes at 25°C. The secondary used was a DAB detection system.

Image courtesy of an anonymous Abreview.

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  Flow Cytometry - ICAM1 antibody [MEM-111] (ab2213)

ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

This image is courtesy of an Abreview submitted by Dr Karppiah Muthumani

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  Western blot - ICAM1 antibody [MEM-111] (ab2213)

All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution

Lane 1 : Western Markers.
Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.

Secondary
Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)

Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible non-specific binding).

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