Overview

  • Product nameAnti-ICAM1 antibody [MEM-111]See all ICAM1 primary antibodies ...
  • Description
    Mouse monoclonal [MEM-111] to ICAM1
  • SpecificityThis antibody reacts with human CD54 antigen.
  • Tested applicationsICC/IF, ELISA, IP, WB, IHC-Fr, Flow Cyt, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).

  • Positive control
    • Sandwich ELISA: supernatant of HUVEC cell line Indirect ELISA: endothelial cell line derived from bovine pulmonary artery RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab2213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use a concentration of 25 µg/ml. Use at a dilution of 1/1000 for Indirect ELISA, 1hr, RT. The antibody reacts with tissue - endothelial cell line derived from bovine pulmonary artery.
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Use under non-reducing conditions.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to ICAM1 (Biotin) (ab7815). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab7815 as Detection.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • CD54 antigen antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody [MEM-111] images

  • Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
  • ab2213 staining ICAM1 in human lung and appendix tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA, pH 8.0, 20 minutes at 100°C. Samples were then incubated with ab2213 at a 1/25 dilution for 20 minutes at 25°C. The secondary used was a DAB detection system.

    See Abreview

  • ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

    See Abreview

  • All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution

    Lane 1 : Western Markers.
    Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
    Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.

    Secondary
    Rabbit Anti-Mouse IgG H&L (HRP) (ab6728)

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-ICAM1 antibody [MEM-111] (ab2213)

This product has been referenced in:
  • Narita T  et al. The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure. Mol Ther 21:860-7 (2013). Rat . Read more (PubMed: 23358187) »
  • Scott DW  et al. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. J Am Heart Assoc 2:e000263 (2013). ELISA ; Human . Read more (PubMed: 23900214) »

See all 8 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Rhesus monkey Cell lysate - whole cell (RF/6A cells from ATCC)
Specification RF/6A cells from ATCC
Treatment 5.5 mM glucose, 30 mM glucose, 30 mM glucose + 0.1 mM BAPN for 10 days
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 26 2014

I sorry to hear about the volume of the antibody; we always fill vials 4-5ul extra so quick spin is required to extract the whole volume. I am sure you have given a quick spin to vial before opening it, if it hasn't been done then it will worth to try....

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (rat cortex (injured))
Specification rat cortex (injured)
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton X-100 in blocking buffer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2013

Thank you for your enquiry.

As the Swiss-Prot database highlights, the mature ICAM1 protein is heavily N-Glycosylated. There at least 9 potential site for this type of post-translational modification which can significantly alter the band siz...

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Thank you for your response.

Could you please specify the size of the non-specific bands? Are they lower or higher than the expected size (58 kDa)? Could you attach an image (saved as a jpeg file) to your e-mail so that I can have a look at t...

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Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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Thank you for calling us yesterday and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mesenchymal stem cell)
Specification Mesenchymal stem cell
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Mr. Steven Johnstone

Verified customer

Submitted Sep 05 2012

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"