Anti-ICAM1 antibody [MEM-111] (ab2213)

Overview

• Product nameAnti-ICAM1 antibody [MEM-111]See all ICAM1 primary antibodies ...
• Description
  Mouse monoclonal [MEM-111] to ICAM1
• SpecificityThis antibody reacts with human CD54 antigen.
• Tested applicationsICC/IF, ELISA, IP, WB, IHC-Fr, Flow Cyt, Sandwich ELISA, IHC-P more details
• Species reactivity
  Reacts with: Rat, Cow, Human
  
• Immunogen

  Tissue / cell preparation (Human). (Burkitt's lymphoma cell line RAJI).

• Positive controlSandwich ELISA: supernatant of HUVEC cell line Indirect ELISA: endothelial cell line derived from bovine pulmonary artery RAJI cell line,K562 leukemia cell line, JY cell line, Activated T-lymphocytes, Thymus, RE cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• Purity>95% by SDS-PAGE
• Purification notesPurified from ascities by protein-A affinity chromatography.
• Clonality Monoclonal
• Clone numberMEM-111
• Myelomaunknown
• IsotypeIgG2a
• Light chain typeunknown
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • T Lymphocytic Lineage

  • Stem Cells
  • Hematopoietic Progenitors
  • Lymphoid
  • B Lymphocytic Lineage

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Cell adhesion molecules

  • Immunology
  • Adaptive Immunity
  • Regulatory T Cells

  • Immunology
  • Cell Type Markers
  • CD
  • Adhesion

Applications

Target

• FunctionICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
• Sequence similaritiesBelongs to the immunoglobulin superfamily. ICAM family.
  Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
• Post-translational
  modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
• Cellular localizationMembrane.

Target information above from: UniProt accession P05362 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3383 Human
  • Entrez Gene: 25464 Rat
  • Omim: 147840 Human
  • SwissProt: P05362 Human
  • SwissProt: Q00238 Rat
  • Unigene: 643447 Human
  • Unigene: 12 Rat

• Alternative names
  Antigen identified by monoclonal antibody BB2 antibodyBB 2 antibodyBB2 antibody

  CD 54 antibodyCD_antigen=CD54 antibodyCD54 antibodyCD54 antigen antibodyCell surface glycoprotein P3.58 antibodyHuman rhinovirus receptor antibodyICAM 1 antibodyICAM-1 antibodyICAM1 antibodyICAM1_HUMAN antibodyintercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibodyIntercellular adhesion molecule 1 antibodyMajor group rhinovirus receptor antibodyMALA 2 antibodyMALA2 antibodyMyD 10 antibodyMyD10 antibodyP3.58 antibodySurface antigen of activated B cells antibodySurface antigen of activated B cells, BB2 antibody

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Anti-ICAM1 antibody [MEM-111] images

• 

  Sandwich ELISA - ICAM1 antibody [MEM-111] (ab2213)
  Standard Curve for ICAM1 (Analyte: ab82125) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-111] to ICAM1 (ab2213) at 5ug/ml and Detector Antibody Rabbit polyclonal to ICAM1 (Biotin) (ab7815) at 0.5ug/ml
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [MEM-111] (ab2213)Image courtesy of an anonymous Abreview.
  ab2213 staining ICAM1 in human lung and appendix tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  
  Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA, pH 8.0, 20 minutes at 100°C. Samples were then incubated with ab2213 at a 1/25 dilution for 20 minutes at 25°C. The secondary used was a DAB detection system.

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• 

  Flow Cytometry - ICAM1 antibody [MEM-111] (ab2213)This image is courtesy of an Abreview submitted by Dr Karppiah Muthumani

  ab2213 staining human PBL cells by flow cytometry.  Cells were formaldehyde fixed and gated on B-cells.  The primary antibody was incubated with the sample for 30 minutes at 25°C.  The secondary antibody was ab6810 (a FITC conjugated chicken polyclonal to mouse IgG - H&L).  Both antibodies were used undiluted.

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• 

  Western blot - ICAM1 antibody [MEM-111] (ab2213)
  All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213) at 1/3000 dilution
  
  Lane 1 : Western Markers.
  Lane 2 : Whole cell lysate prepared from human HUVEC cells (untreated) at 100,000 cells.
  Lane 3 : Whole cell lysate prepared from human HUVEC cells (treated with 1 ng/ml TNF-alpha for 24 h) at 100,000 cells.
  
  Secondary
  Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6728)
  
  Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 40 kDa (possible non-specific binding).

  This image is courtesy of an anonymous Abreview

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