ICAM1 antibody [YN1/1.7.4] (ab25375)

Product Name 

ICAM1 antibody [YN1/1.7.4]

Product type 

Primary antibodies

Description 

Rat monoclonal [YN1/1.7.4] to ICAM1

Immunogen 

The details of the immunogen for this antibody are not available.

Reacts with 
(species key)

Ms

Tested applications 
(see key)

BL, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
BL: Use at an assay dependent dilution (see Takei et al).
IHC-Fr: Use at an assay dependent dilution (see Qin et al).
IP: Use at an assay dependent dilution (see Horley et al).
WB: Use at an assay dependent dilution. Detects a band of approximately 95 kDa (predicted molecular weight: 85-110 kDa) (see Welder et al).

FACS can be performed with conjugated versions of this product (e.g ab24869).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PMA or LPS stimulated T-cell line such as EL-4 or 18-81

Cellular localization 

Type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion

Relevance 

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

YN1/1.7.4

Isotype 

IgG2b

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: None
Constituents: 100mM borate buffered saline. pH 8.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C.

References for ICAM1 antibody [YN1/1.7.4] (ab25375)

  This product has been used in: (two most recent references)

Moussa O et al. Novel role of thromboxane receptors beta isoform in bladder cancer pathogenesis. Cancer Res 68:4097-104 (2008). WB; Human. PubMed: 18519668

Qin L et al. The roles of CD11/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice. J Immunol 157:5016-21 (1996). PubMed: 8943409

See all 5 publication references for this product.


If you publish research using ab25375 please let us know so that we can cite the reference on this datasheet.

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