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Western blot - ICAM1 antibody (ab27536)
(enlarge)
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Product Name
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ICAM1 antibody
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See all ICAM1 antibodies (44)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ICAM1
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Immunogen
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Recombinant fragment, corresponding to amino acids 253-367 of human ICAM1.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody recognises ICAM1.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at room temperature. Predicted molecular weight: 85-110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Raji cells and tonsil for Western blotting.
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Cellular localization
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Type I membrane protein.
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Research areas
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Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Relevance
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ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody is affinity purified.
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ICAM1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ICAM1 antibody images, references, products related to ab27536 and other tools.
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ICAM1 antibody images:
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 Western blot - ICAM1 antibody (ab27536)
All lanes : ICAM1 antibody (ab27536) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HUVEC whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated goat anti-rabbit
Predicted band size : 85-110 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted by Ms Leandra-Louise Bowers
See Abreview
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Search PubMed (MEDLINE) for references to ICAM1
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ICAM1 antibody - more information
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Customer reviews (feedback) regarding ICAM1 antibody |
Customer FAQs regarding ICAM1 antibody |
Protocols for ICAM1 antibody |
Price and availability of products related to ICAM1 antibody |
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ICAM1 antibody - related products:
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ICAM1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ICAM1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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