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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - ICAM1 antibody (ab66100)
All lanes : Anti-ICAM1 antibody (ab66100) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
ICAM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 115 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ICAM1.
Anti-ICAM1 antibody for Western blot in Monkey (66100)
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