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Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM1 antibody
See all ICAM1 products (46) ...
Description
Rabbit polyclonal to ICAM1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human ICAM1.
(Peptide available as ab74481.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Raji, SW480, Jurkat, U2OS
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
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Western blot - ICAM1 antibody (ab66100)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 115 kDa (predicted molecular weight: 58 kDa).
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Anti-ICAM1 antibody images:
Western blot - ICAM1 antibody (ab66100)
All lanes : Anti-ICAM1 antibody (ab66100) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
ICAM1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 115 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to ICAM1.
References for Anti-ICAM1 antibody (ab66100)
ab66100 has not yet been referenced specifically in any publications.
Publishing research using ab66100? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"