Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Cell Type Markers >> CD >> Adhesion
Overview
Product name
Anti-ICAM1 antibody
See all ICAM1 products (46) ...
Description
Rabbit polyclonal to ICAM1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A peptide mapping at the C-terminal region of the human ICAM-1 identical to the related rat and mouse sequence
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab67345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 58 kDa.
IHC-P: Use a concentration of 1 - 2 µg/ml.
Target
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Cellular localization
Membrane.
Target information above from: UniProt accessionP05362
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BB 2 antibody
- BB2 antibody
- CD 54 antibody
see all
Database links
- Entrez Gene: 3383 Human
- Omim: 147840 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Anti-ICAM1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody (ab67345)
ab67345 at 1µg/ml staining ICAM1 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
Western blot - ICAM1 antibody (ab67345)
All lanes : Anti-ICAM1 antibody (ab67345) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human MM231 cells.
Lane 2 : Whole cell lysates prepared from Hela cells.
Lane 3 : Whole cell lysates prepared from SW620 cells.
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size : 58 kDa
References for Anti-ICAM1 antibody (ab67345)
ab67345 has not yet been referenced specifically in any publications.
Publishing research using ab67345? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"